Cross-Link Formation and Peptidoglycan Lattice Assembly in the FemA Mutant ofStaphylococcus aureus
نویسندگان
چکیده
منابع مشابه
Cross-Link Formation and Peptidoglycan Lattice Assembly in the FemA Mutant of Staphylococcus aureus
Staphylococcus aureus FemA mutant grown in the presence of an alanine-racemase inhibitor was labeled with d-[1-(13)C]alanine, l-[3-(13)C]alanine, [2-(13)C]glycine, and l-[5-(19)F]lysine to characterize some details of the peptidoglycan tertiary structure. Rotational-echo double-resonance (REDOR) NMR of isolated cell walls was used to measure internuclear distances between (13)C-labeled alanines...
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The femAB operon codes for two nearly identical approximately 50-kDa proteins involved in the formation of the staphylococcal pentaglycine interpeptide bridge. Sequencing and analysis of the femA region of mutants isolated by chemical mutagenesis and selection for lysostaphin resistance revealed point mutations leading to the expression of truncated FemA proteins. These femA mutants, although s...
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The biosynthesis of the cross-linking peptides in the cell wall peptidoglycan of Staphylococcus aureus.
The biosynthesis of pentaglycine, the cross-linking peptide in the cell wall peptidoglycan of Sfaphylococcus aureus, requires glycyl-tRNA as a donor of glycine. The pentaglycine is introduced by sequential addition of glycine from glycyltRNA to the e-amino group of the L-lysine residue of the pentapeptide in the lipid-linked precursor. The direction of elongation of the pentaglycine chain is fr...
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ژورنال
عنوان ژورنال: Biochemistry
سال: 2014
ISSN: 0006-2960,1520-4995
DOI: 10.1021/bi4016742